Dr. Mohana Kumar Patel
Centre For Ayurveda Biology and Holistic Nutrition
Dr. Mohan completed his Postgraduate and doctoral studies in University of Agricultural Sciences GKVK, Bengaluru-65 under supervision of Prof. Uma Shaanker R. Later, did his postdoctoral Fellow/SERB-DST Young Scientist at Indian Institute of Technology Madras, Chennai, Department of Chemistry under the mentorship of Prof. T Pradeep. He has been trained in the art of conceptual thinking and asking precise questions. These have been the cornerstone of his foundation in research. Past 15 years, he has been trained in tools ranging from biochemical (like HPLC, LCMS/MS, MALDI-TOF, DESI MSI) to molecular techniques (like, DNA markers, genome and transcriptome sequencing, multi-omics application in medicinal plant research).
Expertise & Achievements:
- Associate Professor at TDU, Bengaluru (from January)
- Assistant professor at TDU, Bengaluru (August 2017 to Dec, 2020)
- SERB Young Scientist, IIT Madras Chennai (Feb 2015 to July 2017)
- Institute Postdoctoral Fellow, IIT Madras, Chennai ( April 2014 to March 2017)
- Research Associate, UAS, GKVK, Bengaluru (October 2011 to March 2014)
- Visiting Research Fellow, NCL, Pune, Dept. of Organic Chemistry (August 2011 to September 2011)
- Research Associate, UAS, GKVK, Bengaluru (June 2010 to July 2011)
- Visiting Research Fellow, IISc, Dept. of Organic Chemistry, Mentor: Prof. A. Srikrishna (March 2010 to May 2010)
- Visiting Scholar, Concordia University, Montreal, Canada, , Dept. of Biology, Mentor: Prof. S. Dayanandan (September 2010 to Feb 2011)
- Analytical and natural product chemistry: Isolation, purification and structural characterization of secondary metabolites using ESI MS fragmentations. Semi-synthesis of natural product analogues. Analysis of metabolites using chromatographic techniques like, TLC, HPLC, GC-MS, LC-MS/MS, HRMS/MS and DESI MS
- Molecular biology and bioinformatics: Experience in RNA isolation, Synthesis of cDNA. PCR, RT-PCR, qPCR, cloning and transformation, SSR genotyping using LI-COR automated sequencer. Data analysis using various bioinformatics’ tools (Population genetic, Molecular Phylogenetics and next generation sequencing). DNA barcoding of plants using rbcL,matk, and trnH-psbA etc. Metabolic pathway prediction and data integration
- Microbiology: Isolation of endophytic fungi from plants, their taxonomic characterization, DNA barcoding using rDNA ITS locus/LSU etc., evaluating their secondary metabolite profile using HPLC and LC-MS, ESI MS and HRMS/MS, Gene transformation in bacteria and fungi.
- Organised Indian Academy sponsored refresher course for three consecutive years, 2018,2019,2020 on “multiomics application in medicinal plant research” Course Coordinator
- Virtual lecture workshop / one Credit course (15hr) on “Unravelling the metabolic diversity using multi-omic technologies, 7-12th October, 2020, held at TDU, Bengaluru: Course Coordinator
- Development of curriculum B. Sc and M.Sc In Biological Sciences (Bioinformatics and Functional Genomics).
- Developed the SOPs for the labs and safety guidelines.
- Involved in establishing various labs at TDU like instrumentation lab, bioinformatics, molecular biology, chemistry and analytical labs etc.
Currently, our group at TDU is focused on:
- metabolite imaging and deciphering the metabolic pathways in plant and microbes,
- chemical ecology and boundary chemistry,
- bioprospecting of high values metabolites and drug discovery
- molecular signatures of endemic and endangered medicinal plants;
- sustainable production and use medicinal plants
- plant extract library and drug discovery
- dissecting scientific basis of Ayurveda formulations towards developing super drugs; Yogavahini concept: bio- assimilation/bio-enhancer of Ayurveda formulations/foods towards developing super foods/drugs, etc.
Education interests: M.Sc. Life science in Ayurveda biology, M.Sc. in Biotechnology and Bioinformatics
Outreach programs: Research based seminars/webinars for students, teachers and faculty on multi-omic application in medicinal plant research
Ongoing Research Projects / Grants / Activities:
- “Multi-omic approaches and mass spectrometry based tissue metabolite imaging towards understanding metabolites localisation and metabolic pathways in specific cell/tissues of key medicinal plant, Dysoxylum binectariferum” PI _SERB ECR Project_ 30th March 2019 to 29th March 2022
- Development of sustainable technology for the production of anticancer chromone alkaloids, rohitukine and dysoline from Indian forest trees (Co-ordinator and PI- DBT) Oct 17, 2019 to 16 October, 2022, Collaborated with College of Sirsi, UAS, Dharwad
- “Development of SSR markers and assessment of genetic diversity of natural populations of Pterocarpus santalinus L.f. distributed in the Eastern Ghats, India” total budget (budget: Co-PI, NBA, Gov. of India) september 1, 2020 to August 31 2022. Collaborated with IWST, Bengaluru.
- Functional molecules in Honey for the management of Diabetes and obesity PI, (Jan 2019- Jan 2021)
Publications and Patents
Mass spectrometry imaging; metabolic pathways
- Mohana Kumara P, Srimany A, Arunan S, Ravikanth G, Uma Shaanker R, Pradeep T. 2016 Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F. PLoS ONE 11(6): e0158099. doi:10.1371/journal.pone.0158099
- Mohana Kumara P, Amitava Srimany, Ravikanth G, Uma Shaanker, R. Pradeep, T.2015 Ambient ionization mass spectrometry imaging of rohitukine, a chromone anti-cancer alkaloid, during seed development in Dysoxylum binectariferum Hook. F (Meliaceae). Phytochemistry. Volume 116, 104-110. IF: 3.35
High value metabolites from plant and microbial source:
- Mohana Kumara P, et al. 2014. Rohitukine, a Chromane alkaloid and a precursor of flavopiridol, is produced by endophytic fungi isolated from Dysoxylum binectariferumHook.f and Amoora rohituka (Roxb). Wright &Arn. Mar Phytomedicine15; 21(4): 541-546.
- Mohana kumara P et al. 2010. Dysoxylum binectariferum Hook.f (Meliaceae), a rich source of rohitukine. Fitoterapia. Volume 81, Issue 2:145-148.
- Shweta S, Sebastian Z, Ramesha BT, Priti V, Mohana Kumara P et al. 2010, Endophytic fungal isolates from Apodytes dimidiata E. Mey. exArn (Icacinaceae) produce camptothecin, 10-hydroxycamptothecin and 9-methoxycamptothecin Phytochemistry 71: 117-122.
- Shweta, S, HimaBindu J, Raghu, J, Suma H.K, Manjunath, J, Mohan Kumara, P, et al. 2013 Isolation of endophytic bacteria from Miquelia dentata Bedd., producing the anti-cancer alkaloid, camptothecine. Phytomedicine. 20 (2013) 913– 917
- Suma, H. K., Kumar, V., Senthilkumar, U., Mohana Kumara, P, et al. 2014. Pyrenacantha volubilis Wight, (Icacinaceae) a rich source of camptothecine and its derivatives, from the Coromandel Coast forests of India. Fitoterapia 97, 105-110.
- Vasanthakumari, M. M. Jadhav, S. S. Sachin, N. Vinod, G. Manjunatha, B.L Mohana Kumara, P. Ravikanth, G and Uma Shaanker R. 2015. Passage through host tissue and DNA methyltransferase inhibitor restores CPT production in attenuated endophytic fungus. World J Microbiol Biotechnol. DOI 10.1007/s11274-015-1916-0
- Hemmilä S, Mohana Kumara P et al. 2010. Development of polymorphic microsatellite loci in the endangered tree species Dysoxylum malabaricum, Molecular Ecology Resources 10(2): 404-408
- Sumangala R. C., Mohana Kumara P et al . 2013 Development and characterization of microsatellite markers for Dysoxylum binectariferum, a medicinally important tree species in Western Ghats, India. J. Gen et. 92, e85–e88. Online only: http://www.ias.ac.in/jgenet/OnlineResources/92/e85.pdf]
- Sofia Bodare, G Ravikanth, A Sascha, Mohana Kumara P, et al. 2016 Landscape and fine-scale genetic structure of white cedar (Dysoxylum malabaricum) in disturbed forest patches of the Western Ghats, India. Conservation genetics DOI 10.1007/s10592-016-0877-7.
- “NEW CHROMONE ALKALOID DYSOLINE FOR THE TREATMENT OF CANCER AND INFLAMATORY DISORDERS” Indian Patent (1077DEL2013 (Prov. Date: 10/04/2013) and US Patent 20,160,046,611, 2016
- Mohana Kumara, P, et al. 2013 “Endophytes and plant secondary metabolite synthesis: molecular and evolutionary perspectives” Springer India, pp.177-190. Book edited by Verma, Vijay; Gange, Alan C. (Eds.) dio; 10.1007/978-81-322-1575-2_9
- Canadian Commonwealth Exchange Program, Asia-Pacific (formerly GSEP) 2009-10 to do part of PhD research work at Concordia University, Montreal, Canada under the supervision of Dr. Dayanandan, S.
- Department of Science Technology (DST) sponsored Travel Grant Award, 1-6 August 2010 to attend IMC9, Edinburgh, UK and SERB travel grant to attend the EMBO | EMBL Symposium: Multiomics to Mechanisms – Challenges in Data Integration, 11- 13 September 2019 in EMBL Heidelberg, Germany
- Institute Postdoctoral Fellowship-2014 by Indian institute of Technology, IIT Madras, Chennai.
- SERB-DST- Young Scientist Award (No. SERB/LS-1073/2013)
- ECR SERB GRANT 2019
Membership of Professional Bodies
- Life Member, Society for Biological Chemists (SBC), IISc, Bangalore, India
- Life Member, Indian Society for Mass Spectrometry (ISMAS), Mumbi, India
- UAS, GKVK, Bengaluru
- IWST, Bengaluru.
- College of Sirsi, UAS, Dharwad
- ATREE, Bengaluru
- Department of Biotechnology, Government of India
- SERB, Government of India
- DST, Government of India
- NBA, Government of India
- Dabur India
- Indian Academy of Sciences, Bengaluru
- Ms. Renuka Joshi, SRF
- Ms. Pooja Sinha, JRF
- Ms. Ashwini S, JRF
- Ms. Senha M V, PhD Student
- Mr. Tapas Ranjan Samal, PhD Student
PDF (own fellowship), PhD and Internship positions are available interested can contact to firstname.lastname@example.org